Enzymatic hexosaminidation of lactose

An enzyme, comprising β-N-acetylhexosaminidase having an amino acid sequence identity of at least 70% to SEQ ID NO:

• 1 and carrying at least one mutation selected from the group of mutations consisting of:
• a mutation at position 746 of SEQ ID NO:
• 1;
• a mutation at position 827 of SEQ ID NO:
• 1, preferably carrying a glutamic acid or an alanine or a glutamine;
• a mutation at position 746 of SEQ ID NO:
• 1 and/or carrying a phenylalanine at a position counted as position 827 of SEQ ID NO:
• 1, or a β-N-acetylhexosaminidase comprising an amino acid sequence selected from the group consisting of:
• any-oUSEQ ID NO[[s]]:
• 5 to 8 or comprising an amino acid sequence having an amino acid sequence identity of at least 70% to any of SEQ ID NOs:
• 5 to 8.

A Lacto-N-biosidase having an amino acid sequence identity of at least 70% to SEQ ID NO:

• 3 and carrying at least one of the following substitutes selected from the group consisting of:
• a mutation at a position counted as position 320 of SEQ ID NO:
• 3, a mutation at a position counted as position 419 of SEQ ID NO:
• 3, a glutamic acid or an alanine at a position counted as position 320 of SEQ ID NO:
• 3, a phenylalanine at a position counted as position 419 of SEQ ID NO:
• 3;
• or
• At least one lacto-N-biosidase comprising an amino acid sequence selected from the group consisting of;
• SEQ ID NO:
• 9, SEQ ID NO:10, SEQ ID NO:
• 11.
• and a sequence having an amino acid sequence identity of at least 70% to any of SEQ ID NO:
• 9, SEQ ID NO:10, SEQ ID NO:
• 11.