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US 2022/0252572 A1Published application
Methods and compositions for assembly of biological nanopores
AssigneeStratos Genomics, Inc.
InventorsRobert Busam, Cynthia Cech
Life of this patent
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Abstract
Methods and compositions for the manufacture and use of a detection apparatus based on one or more native biological nanopores are provided. Uses include, but are not limited to, detection and sequencing of nucleic acids.
Claims15
A method of making a detection apparatus comprising one or more native nanopore proteins, comprising the steps of:
- (a) forming an aqueous mixture comprising a nanopore protein, a membrane scaffold protein (MSP), and a first lipid to produce a sample of nanodisc-nanopore protein complexes, wherein a population of the nanodisc-nanopore protein complexes in the sample each comprise a native nanopore protein;
- (b) providing a solid support comprising one or more apertures, wherein a membrane is formed over each of the apertures, wherein the membrane comprises a second lipid, and wherein the membrane separates a cis chamber from a trans chamber in the detection apparatus;
- and
- (c) contacting the one or more membranes with the population of nanopore-nanodisc complexes comprising the native nanopore protein to assimilate a native nanopore protein into each of the membranes.
1 more element
A method of forming a native nanopore protein in a membrane comprising the steps of:
- (a) forming an aqueous mixture comprising a nanopore protein, a membrane scaffold protein (MSP), and a first lipid to produce a sample of nanodisc-nanopore protein complexes, wherein a population of the nanodisc-nanopore protein complexes each comprise a native nanopore protein;
- (b) providing a membrane comprising a second lipid;
- and
- (c) contacting the membrane with the population of nanopore-nanodisc complexes comprising the native nanopore protein to assimilate a native nanopore protein the membranes.
1 more element
Dependencies are inferred from the claim text.