Encapsulated pre-analytic workflows for flow-through devices, liquid chromatography and mass spectrometric analysis
AssigneeWaters Technoligies Corporation
InventorsKevin Daniel Wyndham, Darryl W. Brousmiche
Life of this patent
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Abstract
This invention relates to encapsulated reagents for sample and workflow preparation prior to chromatographic, spectroscopic or other analytical systems, use thereof, and devices comprising the same.
Claims18
An encapsulated workflow reagent comprising an encapsulating material and a workflow reagent encapsulated within the encapsulating material, wherein the workflow reagent encapsulated within the encapsulating material is attached to a surface of a scaffolding material, wherein the scaffolding material is a non-porous solid, wherein the workflow reagent is an enzyme, and wherein the encapsulating material is made with one or more polymers configured to provide a controlled release of the workflow reagent, wherein the encapsulating material is covalently attached to a surface of the scaffolding material and wherein the encapsulated workflow reagent material has the formula [(B)-(Y)n)]o-EM (Formula I), where
- EM represents the encapsulating material;
- B represents a-the scaffolding material;
- Y is a linker group between the encapsulating material and a surface of the scaffolding material;
- o is an integer greater than 0;
- and
- n is an integer greater than or equal to 1.
2-7. (canceled)
The encapsulated workflow reagent according to claim 7, wherein the linker group is of the formula represented by Formula II
- wherein
- n1 an integer from 0-30;
- n2 an integer from 0-30;
- each occurrence of R1, R2, R3 and R4 independently represents hydrogen,
- fluoro, methyl, ethyl, n-butyl, t-butyl, i-propyl, lower alkyl, a protected or
- deprotected alcohol, a zwiterion, or a group Z;
- Z represents:
- a) a surface attachment group having Formula III:
- (B1)x(R5)y(R6)zSi— Formula III:
- wherein x is an integer from 1-3,
- y is an integer from 0-2,
- z is an integer from 0-2,
- and x+y+z=3
- each occurrence of R5 and R6 independently represents methyl, ethyl, n-butyl, iso-butyl, tert-butyl, iso-propyl, thexyl, substituted or unsubstituted aryl, cyclic alkyl, branched alkyl, lower alkyl, a protected or deprotected alcohol, or a zwiterion group;
- B1 represents —OR7, —NR7′R7″, —OSO2CF3, or —Cl;
- where each of R7, R7′ and R7″ represents hydrogen, methyl, ethyl, n-butyl, iso-butyl, tert-butyl, iso-propyl, thexyl, phenyl, branched alkyl or lower alkyl;
- b) an attachment to a surface group through a direct carbon-carbon bond formation or through a heteroatom, ester, ether, thioether, amine, amide, imide, urea, carbonate, carbamate, heterocycle, triazole, or urethane linkage;
- or
- c) an adsorbed, surface group that is not covalently attached to the surface of the material;
- Y′ represents a direct bond;
- a heteroatom linkage;
- an ester linkage;
- an ether linkage;
- an thioether linkage;
- an amine linkage;
- an amide linkage;
- an imide linkage;
- a urea linkage;
- a thiourea linkage;
- a carbonate linkage;
- a carbamate linkage;
- a heterocycle linkage;
- a triazole linkage;
- a urethane linkage;
- a diol linkage;
- a polyol linkage;
- an oligomer of styrene, ethylene glycol, or propylene glycol;
- a polymer of styrene, ethylene glycol, or propylene glycol;
- a carbohydrate group, a multi-antennary carbohydrates, a dendrimer or dendrigraphs, or a zwitterion group;
- and
- A represents attachment to the encapsulating material by a ionic group, non-covalently attachment group or by a direct bond including (but not limited to):
- a heteroatom linkage;
- an ester linkage;
- an ether linkage;
- an thioether linkage;
- an amine linkage;
- an amide linkage;
- an imide linkage;
- a urea linkage;
- a thiourea linkage;
- a carbonate linkage;
- a carbamate linkage;
- a heterocycle linkage;
- a triazole linkage;
- a urethane linkage;
- a diol linkage;
- a polyol linkage;
- an oligomer of styrene, ethylene glycol, or propylene glycol;
- a polymer of styrene, ethylene glycol, or propylene glycol;
- a carbohydrate group, a multi-antennary carbohydrates, a dendrimer or dendrigraphs, or a zwitterion group.
(canceled)
Dependencies are inferred from the claim text.