Acoustic affinity cell selection for multiple target receptors
AssigneeFloDesign Sonics, Inc.
InventorsRui Tostoes, Bart Lipkens, Kedar C. Chitale, Benjamin Ross-Johnsrud, Walter M. Presz, JR., Jack Saloio
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Abstract
Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.
Claims20
A separation system, comprising:
- a vertically oriented column with an opening on each end to permit a fluid flow therethrough, a portion of the column being configured as a fluidized bed in which a plurality of support structures that are targeted to at least two different types of cellular material;
- an acoustic transducer on one end of the column for generating an acoustic standing wave in the column;
- and
- the acoustic transducer being configured to generate the acoustic standing wave to block and retain the support structures in the column against the fluid flow.
A method for separating materials, comprising:
- providing support structures in a column configured as a fluidized bed for binding with at least two different types of cellular material;
- flowing a fluid mixture that includes the at least two different types of cellular material into the column;
- and
- generating an acoustic standing wave with an acoustic transducer near an end of the column to block the support structures from leaving the column with the fluid flow.
An acoustic affinity separation method, comprising:
- providing first affinity beads targeted to a first cellular material in a fluid to a first column;
- providing second affinity beads targeted to a second cellular material in a fluid to a second column;
- generating an acoustic standing wave with an acoustic transducer near an end of each of the first column and the second column;
- flowing a cellular material fluid mixture in the first column and through the acoustic standing wave;
- configuring the acoustic standing wave in the first column to prevent the first affinity beads from passing through the acoustic standing wave;
- flowing the cellular material fluid mixture that passed through the acoustic standing wave in the first column to the second column and through the acoustic standing wave of the second column;
- and
- configuring the acoustic standing wave in the second column to prevent the second affinity beads from passing through the acoustic standing wave of the second column.
Dependencies are inferred from the claim text.