Method for synthesizing lacto-n-biose
AssigneeShandong Henglu Biotech. Co., Ltd.
InventorsXu FANG, Zhiqiang DU
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Abstract
A method for synthesizing lacto-N-biose and belongs to the technical field of bioengineering and oligosaccharide synthesis. A multi-enzyme catalytic system with good biological safety and wide application, and an ATP regeneration cycle system is introduced into a multi-enzyme reaction system, so that the synthesis of lacto-N-biose and the utilization rate of substrates are improved. A novel lacto-N-biose synthetic route lays a foundation for large-scale industrial production of lacto-N-biose and has important economic values and social benefits. At the same time, the synthetic method is efficient, mild, simple, easy to operate, low in cost and suitable for industrial production, and has a high practical application value.
Claims12
A method for synthesizing lacto-N-biose, the method comprising:
- adding galactokinase and lacto-N-biose phosphorylase into a reaction system containing galactose, acetylglucosamine and lactose as substrates to prepare lacto-N-biose;
- and
- adding acetyl phosphate and acetate kinase into the above reaction system for in-situ regeneration of ATP.
Dependencies are inferred from the claim text.