Production of l-2-aminobutyrate from citramalate,citraconate or 2-oxobutanoate
AssigneeAnna University
InventorsAkila T., Padmapriya G., Anitha Janet Roshni Y., Tamilselvan J., Nikhil SANGITH, Mahalakshmi NATARAJAN, Pandiraj SUPPURAM, Ramalingam S.
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Abstract
The present invention relates to preparation of key drug intermediate, L-2-amino butyric acid (L-2-ABA) by a method of cell free system and biotransformation using genetically engineered strains from easily available economic substrates like citramalate or citraconate and enzymes like LeuCD, LeuB and ValDH or IlvE.
Claims12
A process for production of L-2-Aminobutyric acid by an enzymatic biotransformation of a substrate selected from citramalate or citraconate in a cell free system wherein the enzymes are isopropyl malate isomerase, isopropylmalate dehydrogenase and valine dehydrogenase.
A process to produce L-2-Aminobutyric acid by whole cell biotransformation inside a whole cell or permeabilized cell of a suitable host of a substrate wherein the substrate is selected from citramalate or citraconate wherein the enzymes are isopropyl malate isomerase, isopropylmalate dehydrogenase and amino acid dehydrogenase or Transaminase B.
Dependencies are inferred from the claim text.