US 2022/0236153 A1 · ANALYTE CONCENTRATOR SYSTEM AND METHODS OF USE

Abstract

Systems and methods for concentrating an analyte preparatory to analysis thereof include processing the effluent of an analyte concentrator to produce an eluent for eluting an analyte retained in the same or separate concentrator, and systems implementing the same. The analyte concentrator system connects the effluent outlet of an analyte concentrator column to an eluent generation module such that the substantially analyte-free effluent discharged from the analyte concentrator column passes fluidly into the eluent generation module. Eluent generated from the substantially analyte-free effluent in the eluent generation module is likewise substantially free of the analyte. The systems and methods can minimize and/or (substantially) eliminate background signal during analysis of the concentrated analyte.

Claims19

Dependent claims

An analyte concentrator system, comprising:

an analyte concentrator assembly, comprising:
a first fluid inlet for receiving a fluid sample comprising an analyte;
an analyte retention mechanism coupled with the first fluid inlet, the analyte retention mechanism being configured to retain at least a portion of the analyte;
a first fluid outlet for discharging an effluent of the fluid sample, the first fluid outlet being coupled with the analyte retention mechanism;
and
a second fluid inlet for receiving an eluent, the analyte retention mechanism being coupled with the second fluid inlet so as to receive the eluent;
and
an eluent generation module coupled with the first fluid outlet and with the second fluid inlet of the analyte concentrator assembly, the eluent generation module being configured to receive the discharged effluent and to generate the eluent from the discharged effluent, the eluent being substantially free of the analyte.

5 more elements

A method of concentrating an analyte, comprising:

processing a first portion of a fluid sample comprising an analyte in a first analyte concentrator assembly, the first analyte concentrator assembly retaining a first portion of the analyte and discharging a first effluent that is substantially free of the analyte, the discharged first effluent being fluid transferred from the first analyte concentrator assembly to an eluent generation module;
processing a second portion of the fluid sample comprising the analyte in a second analyte concentrator assembly, the second analyte concentrator assembly retaining a second portion of the analyte and discharging a second effluent that is substantially free of the analyte, the discharged second effluent being fluid transferred from the second analyte concentrator assembly to the eluent generation module;
processing the transferred first effluent within the eluent generation module so as to produce a first eluent that is substantially free of the analyte from the transferred first effluent, the first eluent being fluid transferred from the eluent generation module to the second analyte concentrator assembly;
processing the transferred second effluent within the eluent generation module so as to produce a second eluent that is substantially free of the analyte from the transferred second effluent, the second eluent being fluid transferred from the eluent generation module to the first analyte concentrator assembly;
eluting at least a portion of the analyte retained by the first analyte concentrator assembly with the second eluent so as to produce a first concentrated analyte sample, the first concentrated analyte sample being fluid transferred from the first analyte concentrator assembly to an analyte detection module;
eluting at least a portion of the analyte retained by the second analyte concentrator assembly with the first eluent so as to produce a second concentrated analyte sample, the second concentrated analyte sample being fluid transferred from the second analyte concentrator assembly to the analyte detection module;
analyzing the first concentrated analyte sample in the analyte detection module;
and
analyzing the second concentrated analyte sample in the analyte detection module.

6 more elements

Dependencies are inferred from the claim text.

Analyte concentrator system and methods of use

Life of this patent

Analyte concentrator system and methods of use

Life of this patent

Abstract

Claims19