Sparger assemblies for a bioprocessing system
AssigneeGlobal Life Sciences Solutions USA LLC
InventorsANDREAS CASTAN, RICHARD DAMREN
Life of this patent
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Abstract
A sparger assembly for a bioprocessing system includes a first layer having a plurality of pores of a first size, and a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size. The pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.
Claims29
A sparger assembly for a bioprocessing system, comprising:
- a first layer having a plurality of pores of a first size;
- and
- a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size;
- wherein the pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.
A bioprocessing system, comprising:
- a vessel;
- a flexible bioprocessing bag positionable within the vessel;
- and
- a sparger assembly positioned at a bottom of the flexible bioprocessing bag, the sparger assembly including:
- a first layer having a plurality of pores of a first size;
- and
- a second layer disposed above the first layer and having a plurality of holes of a second size, the second size being greater than the first size;
- wherein the pores of the first layer and the holes of the second layer allow for the passage of a sparge gas through the first layer and the second layer.
A sparger assembly, comprising:
- a base layer;
- a dielectric layer disposed above the base layer;
- a top layer disposed above the dielectric layer, the top layer having an upper surface;
- at least one electrode in contact with the dielectric layer;
- and
- at least one sparge gas opening in at least the hydrophobic layer for facilitating the formation of a bubble of sparge gas on the upper surface of the top layer for introduction of the sparge gas into a bioreactor vessel.
A bioprocessing system, comprising:
- a vessel;
- a flexible bioprocessing bag positionable within the vessel;
- and
- a sparger assembly positioned at a bottom of the flexible bioprocessing bag, the sparger assembly including:
- a base layer;
- a dielectric layer disposed above the base layer;
- a top layer disposed above the dielectric layer, the top layer having an upper surface;
- at least one electrode in contact with the dielectric layer;
- and
- at least one sparge gas opening in at least the hydrophobic layer for facilitating the formation of a bubble of sparge gas on the upper surface of the top layer for introduction of the sparge gas into a bioreactor vessel.
A method for bioprocessing, comprising the steps of:
- positioning a sparger assembly in a bioreactor vessel, the sparger assembly having a base layer, a dielectric layer disposed above the base layer, a top layer disposed above the dielectric layer, the top layer having an upper surface, at least one electrode in contact with the dielectric layer, and at least one sparge gas opening in at least the hydrophobic layer for facilitating the formation of a bubble of sparge gas on the upper surface of the top layer for introduction of the sparge gas into the bioreactor vessel;
- electrically connecting the at least one electrode to a voltage source;
- and
- adjusting a voltage supplied to the at least one electrode to adjust a diameter of the bubble formed on the upper surface of the top layer.
Dependencies are inferred from the claim text.