Hair Follicle Primordia and Method for Producing Same
AssigneesNational University Corporation Yokohama National University, Kanagawa Institute of Industrial Science and Technology
InventorsJunji Fukuda, Tatsuto Kageyama, Akihiro Shimizu, Rikuma Nakajima, Riki Anakama
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Abstract
Provided is a hair follicle germ capable of forming a hair shaft-like structure in vitro simply and within a short period of time. A method of producing a hair follicle germ includes: inoculating epithelial cells and mesenchymal cells; maintaining the epithelial cells and the mesenchymal cells in a culture solution in which (a) laminin and entactin, and/or (b) type IV collagen is dispersed; and co-culturing the epithelial cells and the mesenchymal cells in a culture solution to form a hair follicle germ.
Claims9
A method of producing a hair follicle germ, comprising:
- inoculating epithelial cells and mesenchymal cells;
- maintaining the epithelial cells and the mesenchymal cells in a culture solution in which (a) laminin and entactin, and/or (b) type IV collagen is dispersed;
- and
- co-culturing the epithelial cells and the mesenchymal cells in a culture solution to form a hair follicle germ.
A method of promoting formation of a hair shaft-like structure in a hair follicle germ in cell culture comprising:
- inoculating epithelial cells and mesenchymal cells;
- and co-culturing the epithelial cells and the mesenchymal cells to form the hair follicle germ,
- the method comprising promoting the formation of the hair shaft-like structure in the hair follicle germ by maintaining the epithelial cells and the mesenchymal cells in a culture solution in which (a) laminin and entactin, and/or (b) type IV collagen is dispersed.
A method of using (a) laminin and entactin, and/or (b) type IV collagen, for promoting formation of a hair shaft-like structure in a hair follicle germ in cell culture comprising:
- inoculating epithelial cells and mesenchymal cells;
- and co-culturing the epithelial cells and the mesenchymal cells to form the hair follicle germ.
A hair follicle germ, comprising epithelial cells and mesenchymal cells,
- wherein the hair follicle germ comprises a hair shaft-like structure, being free of an arrector pili muscle structure and/or a sebaceous gland structure, and not yet being transplanted to a living body.
Dependencies are inferred from the claim text.