Fabrication of a biomimetic platform system and methods of use
OwnerJason A. Spector, Kristy A. Browninventors
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Abstract
The present technology relates to three-dimensional biomimetic platforms for culturing patient specific cells and tissues in biological material that closely recapitulates the native in vivo environment. The platforms of the present technology enable rapid and flexible biochemical, genomic, and metabolic analysis using a wide variety of assays, and live or end-point biological imaging.
Claims30
A three-dimensional biomimetic platform comprising
- (a) a biocompatible substrate including collagen, a stromal vascular fraction, adipocytes, and organoids, and optionally lymphatic endothelial cells;
- and
- (b) patient-specific cells, wherein the patient-specific cells are homogenously or heterogeneously dispersed within the biocompatible substrate.
A three-dimensional biomimetic platform system comprising
- (a) a biocompatible substrate including collagen, wherein the biocompatible substrate comprises one or more conduits, optionally wherein the biocompatible substrate further comprises a stromal vascular fraction, adipocytes, and organoids and optionally lymphatic endothelial cells;
- and
- (b) patient-specific cells cultured in the one or more conduits.
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A method for producing a biomimetic platform system comprising
- (a) preparing a biocompatible substrate;
- (b) embedding a sacrificial material within the biocompatible substrate;
- (c) degrading the sacrificial material to produce one or more conduits within the biocompatible substrate;
- and
- (d) applying patient-specific cells to the one or more conduits within the biocompatible substrate.
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Dependencies are inferred from the claim text.