Separation moieties and methods of use thereof
AssigneeWerewolf Therapeutics, Inc.
InventorsWilliam WINSTON, Luke EVNIN, Vinay BHASKAR, Giselle KNUDSEN, Daniel J. HICKLIN, Cynthia SEIDEL-DUGAN, Jose Andres SALMERON GARCIA, Heather R. BRODKIN
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Abstract
Provided herein are separation moieties that are suitable for use in conjunction with a variety of therapeutic payloads. The separation moieties serve to generate conditionally active macromolecules whereby the macromolecules have reduced or minimal biological activity until the separation moieties are modified under specific conditions.
Claims27
1-74. (canceled)
A recombinant pro-protein comprising:
- a. a recombinant polypeptide comprising, a cleavable moiety that is a substrate for a protease, wherein the cleavable moiety comprises the amino acid sequence of SEQ ID NO:
- 198, SEQ ID NO:
- 199, SEQ ID NO:
- 381 or an amino acid sequence at least at least 85% identical to the amino acid sequence of SEQ ID NO:
- 198;
- SEQ ID NO:
- 199, SEQ ID NO:
- 381;
- and
- b. a polypeptide with biological activity.
The recombinant pro-protein, wherein the polypeptide with biological activity comprises a cytokine, chemokine, growth factor, a soluble receptor, antigen-binding polypeptide, an antibody or an antigen-binding portion thereof, or a combination thereof.
A nucleic acid composition comprising one or more nucleic acids encoding a recombinant pro-protein comprising:
- a. a recombinant polypeptide comprising, a cleavable moiety that is a substrate for a protease, wherein the cleavable moiety comprises the amino acid sequence of SEQ ID NO:
- 198, SEQ ID NO:
- 199, SEQ ID NO:
- 381, or an amino acid sequence at least at least 85% identical to the amino acid sequence of SEQ ID NO:
- 198, SEQ ID NO:
- 199, SEQ ID NO:
- 381;
- and
- b. a polypeptide with biological activity.
A polypeptide comprising a protease cleavable amino acid sequence of SEQ ID NO:198, SEQ ID NO:
- 199, SEQ ID NO:
- 381 or an amino acid sequence that has at least about 85% identity to SEQ ID NO:198, SEQ ID NO:
- 199, or SEQ ID NO:
- 381.
Dependencies are inferred from the claim text.