Method for producing 2-methyl-butyric acid by bacterial fermentation
AssigneeAjinomoto Co., Inc.
InventorsNatalia Viktorovna Stoynova, Elena Viktorovna Sycheva, Anna Alexandrovna Ublinskaya, Valery Vasilievich Samsonov, Alexandr Dmitrievich Kivero
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Abstract
The present invention provides a method for producing 2-methyl-butyric acid by fermentation using a bacterium belonging to the order Enterobacterales which has been modified to attenuate expression of a tyrB gene encoding a protein having tyrosine aminotransferase activity. The method also allows for production of a byproduct substance of 2-methyl-butyric acid during fermentation of the Enterobacterales bacterium having 2-methyl-butyric acid-producing ability.
Claims12
A method for producing 2-methyl-butyric acid comprising:
- (i) cultivating in a culture medium a 2-methyl-butyric acid-producing bacterium belonging to the order Enterobacterales to produce and accumulate 2-methyl-butyric acid in the culture medium or cells of the bacterium, or both, and
- (ii) collecting 2-methyl-butyric acid from the culture medium or the cells of the bacterium, or both,
- wherein said bacterium has been modified to attenuate expression of a gene encoding a protein having tyrosine aminotransferase activity.
Dependencies are inferred from the claim text.