Back to results
US 2022/0204983 A1Published application
Brassica rod1 gene sequences and uses thereof
AssigneeBASF Agricultural Solutions Seed US LLC
InventorsPeter DENOLF, Michel VAN THOURNOUT, Stephane BOUROT
Life of this patent
Estimating the term…
Abstract
The present invention relates to Brassica juncea ROD1 nucleic acid sequences and proteins and the use thereof to create plants with increased levels of C18:1 and reduced levels of saturated fatty acids in the seeds.
Claims5
1-21. (canceled)
A method for producing oil, comprising
- (a) harvesting seeds from Brassica juncea plants comprising seven ROD1 genes, wherein two ROD1 genes are knock-out ROD1 genes, wherein one knock-out ROD1 gene is a knock-out allele of the ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 2, and wherein one knock-out ROD/gene is a knock-out allele of the ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 4;
- and
- (b) extracting the oil from said seeds.
A method of producing food, feed, or an industrial product comprising
- (a) obtaining a Brassica juncea plant or a part thereof, said Brassica juncea plant comprising seven ROD1 genes, wherein two ROD1 genes are knock-out ROD1 genes, wherein one knock-out ROD1 gene is a knock-out allele of the ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 2, and wherein one knock-out ROD1 gene is a knock-out allele of the ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 4;
- and
- (b) preparing the food, feed or industrial product from the plant or part thereof.
A method for producing oil, comprising
- (a) harvesting seeds from Brassica juncea plants comprising seven ROD1 genes, or a cell, part, seed or progeny thereof, comprising a chimeric gene, said chimeric gene comprising the following operably linked DNA fragments:
- i) a plant-expressible promoter;
- ii) a DNA region, which when transcribed yields an RNA molecule inhibitory to at least two ROD/genes;
- and optionally
- iii) a transcription termination and polyadenylation region functional in plant cells,
- wherein said RNA molecule is inhibitory to a ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 2 and to a ROD1 gene encoding a protein having at least 90% sequence identity to SEQ ID No. 4;
- and
- (b) extracting the oil from said seeds.
5 more elements
Dependencies are inferred from the claim text.