Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
AssigneeNmc S.A.
InventorRichard Thomas Sayre
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Abstract
Embodiments of the present invention provide for a transgenic plan, methods of making and DNA contructs for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chl a/b ratio at high light intensity and a decrease in the Chl a/b ratio at low light intensity versus wild-type plants grown in the same conditions.
Claims16
1-34. (canceled)
A genetically modified plant wherein a native chlorophyll a oxidase gene (Cao) has suppressed Cao expression in the genetically modified plant, the genetically modified plant comprising:
- a DNA construct comprising a heterologous expression control sequence operatively linked to a polynucleotide sequence encoding a chlorophyll a oxidase wherein the expression control sequence interacts with a redox-sensitive modulator responsive to changes in ambient light intensity, wherein the redox-sensitive modulator is chosen from NAB1, GCD2, and GLD-1.
A method to produce a genetically modified plant, the method comprising the steps of:
- a) transforming a plant with a heterologous polynucleotide sequence comprising an expression control sequence operatively linked to a polynucleotide sequence encoding a chlorophyll a oxidase wherein the expression control sequence interacts with a redox-sensitive modulator responsive to changes in ambient light intensity, wherein the redox-sensitive modulator is chosen from NAB1, GCD2, and GLD-1 wherein the native Cao has suppressed Cao expression in the genetically modified plant;
- and
- b) selecting the genetically modified plant that is capable of modulating the antenna size in response to changes in light intensity such that there is an increase in the Chl a/b ratio of the antenna complex composition in the upper canopy (high light intensity) versus a Chl a/b ratio in an antenna complex composition in an upper canopy of wild-type plants grown in the same conditions and a decrease in the Chl a/b ratio in a lower canopy (low light intensity) of the genetically modified plant as compared to the Chl a/b ratio in the upper canopy of the genetically modified plant.
A method to produce a genetically modified plant, the method comprising the steps of:
- a) producing a genetically modified plant wherein an endogenous chlorophyll a oxidase gene (Cao) has suppressed Cao expression;
- b) transforming the genetically modified plant with a heterologous polynucleotide sequence encoding for a modified chlorophyll a oxidase wherein expression of the modified chlorophyll a oxidase is controlled by changes in ambient light intensity wherein the expression control sequence interacts with a redox-sensitive modulator responsive to changes in ambient light intensity, wherein the redox-sensitive modulator is chosen from NAB1, GCD2, and GLD-1;
- and
- c) selecting a genetically modified plant that is capable of modulating the antenna size in response to changes in light intensity such that there is an increase in the Chl a/b ratio of the antenna complex composition in the upper canopy (high light intensity) versus a Chl a/b ratio in an antenna complex composition in an upper canopy of wild-type plants grown in the same conditions and a decrease in the Chl a/b ratio in a lower canopy (low light intensity) of the genetically modified plant as compared to the Chl a/b ratio in the upper canopy of the genetically modified plant.
Dependencies are inferred from the claim text.