Plants and methods for producing 2-pyrone-4, 6-dicarboxylic acid (pdc)
AssigneeThe Regents of the University of California
InventorsChien-Yuan Lin, Aymerick Guillaume Eudes
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Abstract
The present invention provides a genetically modified plant or plant cell comprising a nucleic acid encoding one or more heterologous enzymes operably linked a promoter, wherein one or more heterologous enzymes synthesizes 2-pyrone-4,6-dicarboxylic acid (PDC).
Claims10
A genetically modified plant or plant cell comprising a nucleic acid encoding one or more heterologous enzymes operably linked a promoter, wherein one or more heterologous enzymes synthesizes 2-pyrone-4,6-dicarboxylic acid (PDC).
The genetically modified plant or plant cell of claim 2 comprising one or more nucleic acids encoding protocatechuate 4,5-dioxygenase (PmdAB), or a homologous enzyme thereof, and/or 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (PmdC), or a homologous enzyme thereof, or operably linked to one or more promoters, wherein the genetically modified plant or plant cell is capable of producing protocatechuate (PCA) and produces 2-pyrone-4,6-dicarboxylic acid (PDC).
A genetically modified plant or plant cell comprising one or more nucleic acids encoding protocatechuate 4,5-dioxygenase (PmdAB), or a homologous enzyme thereof, and/or 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (PmdC), or a homologous enzyme thereof, or operably linked to one or more promoters, wherein the genetically modified plant or plant cell is capable of producing protocatechuate (PCA) and produces 2-pyrone-4,6-dicarboxylic acid (PDC). A genetically modified plant or plant cell comprising one or more nucleic acids encoding one or more of the following enzymes:
- 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (AroG), or feedback-resistant DAHP synthase (AroG*), chorismate pyruvate-lyase (UbiC), 3-dehydroshikimate dehydratase (QsuB), p-hydroxybenzoate 3-monooxygenase (PobA), or PobA* (such as a Y385F/T294A PobA mutant), protocatechuate 4,5-dioxygenase (PmdAB), 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (PmdC), protocatechuate 3,4-dioxygenase (PcaGH), and 2-pyrone-4,6-dicarboxylate hydrolase (LigI), or any homologous enzyme of any of the enzymes thereof, operably linked to one or more promoters, wherein the genetically modified plant or plant cell is capable of producing chorismate (CHA) and produces 2-pyrone-4,6-dicarboxylic acid (PDC).
A method for producing a PDC comprising:
- (a) optionally genetically modifying a plant or plant cell to produce a genetically modified plant or plant cell of the present invention, (b) growing or culturing the genetically modified plant or plant cell to produce a PDC, and (c) optionally recovering the PDC produced from the plant or plant cell.
Dependencies are inferred from the claim text.