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AssigneePresident and Fellows of Harvard College
InventorsDavid R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.
1-152. (canceled)
An expression vector comprising a selection substrate linked to a nucleic acid encoding a dominant negative pIII (pIII-neg) protein variant.
A cell comprising the expression vector comprising a first selection substrate linked to a nucleic acid encoding a dominant negative pIII (pIII-neg) protein variant.
Dependencies are inferred from the claim text.