Method and system for visualising colocalised fluorescence signals

A computer system comprising:

• a processor and a memory configured to provide computer program instructions to the processor to execute functions of components:
• an accessing component for accessing fluorescence data in a three-dimensional form including signal intensity data for a first fluorescence channel and a second fluorescence channel associated with each voxel of an image;
• a regression calculating component for calculating a regression on the fluorescence data to generate a regression parameter corresponding to a degree of correlation between the signal intensity data obtained from the first and second fluorescence channels;
• a mapping component for mapping the signal intensity data for each voxel to the regression parameter;
• a colourmap value assigning component for assigning a colourmap value to each voxel based on the mapping;
• and
• a rendering component for rendering the voxels in the image for display on a computer display in colours related to the assigned colourmap values resulting in a visualisation of colocalisation of the fluorescence data in the image.

A computer program product comprising a computer-readable medium having stored computer-readable program code for performing the steps of:

• accessing fluorescence data in a three-dimensional form including signal intensity data for a first fluorescence channel and a second fluorescence channel associated with each voxel of an image;
• calculating a regression on the fluorescence data to generate a regression parameter corresponding to a degree of correlation between the signal intensity data obtained from the first and second fluorescence channels;
• mapping the signal intensity data for each voxel to the regression parameter;
• assigning a colourmap value to each voxel based on the mapping;
• and
• rendering the voxels in the image in colours related to the assigned colourmap values resulting in a visualisation of colocalisation of the fluorescence data in the image.