Method for detecting a cell event
AssigneeCommissariat A L'energie Atomique Et Aux Energies Alternatives
InventorsCédric Allier, Dorothée Krämer, Lionel Herve
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AssigneeCommissariat A L'energie Atomique Et Aux Energies Alternatives
InventorsCédric Allier, Dorothée Krämer, Lionel Herve
Estimating the term…
Method for detecting or predicting the occurrence of a cell event, selected from between cell division or cell death, in a sample (10), the sample extending in at least one sample plane (P10) and comprising cells (10p) immersed in a medium, the method comprising the following steps: a) arranging the sample between a light source (11) and an image sensor (16); b) illuminating the sample (10) using the light source (11) and acquiring a plurality of successive images ((Ii(ti), I1(ti+1) . . . I1(ti+K)) of the sample at different instants ([ti; ti+K]) forming an acquisition time range, each image being representative of an exposure light wave to which the image sensor is exposed; c) on the basis of each acquired image of the sample, calculating an observation image at each instant (I10(ti), I10(ti+1) . . . I10(ti+K)), the observation image corresponding to a spatial distribution of an optical path difference induced by the cells, or of an absorbance of the cells, in the sample plane (P10); d) using the observation images resulting from c) as input data of a supervised artificial intelligence algorithm (CNNd, CNNp), so as to predict or locate an occurrence of the cell event.
A method for detecting or predicting the occurrence of a cell event, selected from between cell division or cell death, in a sample, the sample extending in at least one sample plane and comprising cells immersed in a medium, the method comprising:
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