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US 2022/0187584 A1Published application
Background-suppressed sted nanoscope
AssigneeThe Johns Hopkins University
InventorsTaekjip Ha, Jong-Chan Lee, Ye Ma
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Abstract
The present invention is directed toward a system and method for STED nanography, which reduces background noise. To remove background noise from a STED image, the polarization of the STED beam is altered from that used to obtain the original image. A polarized image is obtained. This polarized image can then be subtracted from the original image to remove noise inherent to the image.
Claims20
A method of stimulated emission depletion (STED) microscopy using background suppression comprising:
- generating from a STED microscope a first image of a specimen and of background noise;
- changing the polarization of a beam from the STED microscope and obtaining a second image of the background noise;
- subtracting the second image from the first image to eliminate the background noise;
- and,
- generating a revised image of the specimen without the background noise.
2 more elements
A system for stimulated emission depletion microscopy using background suppression comprising:
- a stimulated emission depletion (STED) microscope, wherein the STED microscope is configured for:
- generating a first image of a specimen and of background noise;
- changing the polarization of a beam from the STED microscope and obtaining a second image of the background noise;
- and
- a non-transitory computer readable medium programmed for subtracting the second
- image from the first image to eliminate the background noise;
- and,
- generating a final image of the specimen without the background noise.
5 more elements
Dependencies are inferred from the claim text.