High speed scanning systems for super resolution imaging
AssigneeApton Biosystems, Inc.
InventorsWindsor OWENS, Bryan P. STAKER, Robert HARTLAGE, Edvinas ZIZMINSKAS, Paul HEILMAN, Jim JAHNCKE
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Abstract
Disclosed herein is a high throughput optical scanning system to generate super resolution images and methods of use. The optical scanning device and methods of use provided herein can allow high throughput scanning of a continuously moving object with a high resolution despite fluctuations in stage velocity. This can aid in high throughput scanning of a substrate, such as a biological chip comprising fluorophores. Also provided herein are improved optical relay systems and scanning optics.
Claims37
An imaging system comprising:
- a. a light source;
- b. one or more cameras operably coupled to an objective lens to capture a plurality of fields;
- c. a stage configured to support a substrate, wherein said stage is configured to move along a defined path adjacent to said light source;
- d. an encoder mechanically operably coupled to said stage;
- e. a position processing module configured to receive positional information from said encoder and perform a plurality of calculations of a plurality of positions for said stage;
- f. a scanning mirror coupled to an axis adjacent to said defined path operably coupled to said position processing module to receive said plurality of calculations of said plurality of positions for said stage;
- and
- g. a control module configured to operate components (a)-(f) synchronously.
A method of imaging a plurality of fields on a moving substrate utilizing the system of any one of the preceding claims.
A method of imaging a moving substrate, wherein said moving substrate comprises a plurality of fields, comprising:
- a. disposing said substrate on a stage, wherein said stage is configured to move along a defined path adjacent to a light source;
- b. configuring a position processing module to receive positional information from an encoder mechanically operably coupled to said stage, and wherein said position processing module is further configured to perform a plurality of calculations of a plurality of positions for said stage;
- c. configuring a scanning mirror coupled to an axis adjacent to said defined path operably coupled to said position processing module to receive said plurality of calculations of said position for said stage;
- and
- d. imaging at least a field of said plurality of fields with one or more cameras.
Dependencies are inferred from the claim text.