Methods of Terpene Profiling Cannabis Plant Material
OwnerAaron Christopher Elkins, Simone Jane Rochfort +3inventors
InventorsAaron Christopher Elkins, Simone Jane Rochfort, Noel Cogan, German Carlos Spangenberg, Christian Krill
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Abstract
The present disclosure relates to methods of determining the terpene profile of cannabis plant material, specifically through use of gas chromatography-mass spectrometry (GC-MS). Extraction methods include liquid based extraction methods, specifically hexane based liquid extraction methods, and headspace extraction methods, specifically static headspace extraction and headspace solid phase microextraction (HS-SPME). Static headspace and HS-SPME are shown to be better for monoterpene extraction, and hexane based liquid extraction for sesquiterpene extraction.
Claims19
1-27. (canceled)
A method for determining a monoterpene profile of cannabis plant material, the method comprising:
- a. obtaining a reference spectrum of known monoterpenes;
- b. selectively extracting one or more monoterpenes from the cannabis plant material, preferably wherein the plant material comprises one or both of inflorescence and leaf, preferably wherein the plant material is derived from a female cannabis plant;
- c. obtaining spectroscopic data from the monoterpenes separated in step (b);
- d. comparing the spectroscopic data to the reference spectrum to identify and/or measure the level of one or more monoterpenes to determine the monoterpene profile of the cannabis plant material.
A method for determining a sesquiterpene profile of cannabis plant material, the method comprising:
- a. obtaining a reference spectrum of known sesquiterpenes;
- b. selectively extracting one or more sesquiterpenes from the cannabis plant material, preferably wherein the plant material comprises one or both of inflorescence and leaf, preferably wherein the plant material is derived from a female cannabis plant;
- c. obtaining spectroscopic data from the sesquiterpenes separated in step (b);
- d. comparing the spectroscopic data to the reference spectrum to identify and/or measure the level of one or more sesquiterpenes to determine the sesquiterpene profile of the cannabis plant material.
Dependencies are inferred from the claim text.