Use of a ps396 assay to diagnose tauophaties
AssigneeH. Lundbeck A/S
InventorsJan Torleif Pedersen, Thomas Karikari, Kina Höglund, Kaj Blennow, Mikkel Harndahl Harndahl, Henrik Zetterberg
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Abstract
The present invention relates to an in vitro assay for measuring phosphorylated tau in a sample, said assay comprises the use of 2 antibodies i) a capture antibody specific for pS396 on tau and ii) a detection antibody binding tau on a different epitope that the capture antibody.
Claims17
An in vitro assay for measuring phosphorylated tau in a sample, said assay comprises the use of 2 antibodies i) a capture antibody specific for pS396 on tau and ii) a detection antibody binding tau on a different epitope than the capture antibody.
(canceled)
A method for measuring phosphorylated tau in a sample, which method comprises the steps of
- a. Mixing capture antibodies specific for pS396 attached to paramagnetic beads with biotinylated detection antibodies and a sample,
- b. Incubating the mixture at a sufficient time to allow the antibodies to bind to tau in the sample (e.g. 1, 2, 3 or 5 minutes or more),
- c. Optionally, washing the mixture in step b) after incubation,
- d. Adding streptavidin-conjugated beta-galactosidase and allowing said streptavidin-conjugate and the biotinylated detector antibody to react (e.g. 1, 2, 3 or 5 minutes or more),
- e. Optionally washing the obtained mixture after step d),
- f. Adding resorufin beta-D-galactopyranoside to the mixture in step d) or e) and allowing the hydrolysis of resorufin beta-D-galactopyranoside (e.g. 1, 2, 3 or 5 minutes or more),
- g. Reading the fluorescence signal and comparing the signal with a standard.
Dependencies are inferred from the claim text.