A method of identifying a flavivirus infection, and related peptides, kits and compositions
AssigneeAgency for Science, Technology and Research
InventorsFong Poh Lisa Ng, Siti Naqiah Amrun, Wearn Xin Yee
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Abstract
There is provided a method of identifying a flavivirus infection selected from Zika virus (ZIKV), Dengue virus (DENV) and combination thereof in a subject, the method comprising determining whether a sample of the subject reacts with a peptide associated with a certain relative binding capacity. Also claimed are kits, isolated peptides, immune system stimulating compositions comprising specific peptides and a method of distinguishing ZIKV infection from DENV infection.
Claims21
A method of identifying a flavivirus infection selected from Zika virus ZIKV), dengue virus (DENV) and combination thereof in a subject, the method comprising:
- determining whether a sample of the subject reacts with a peptide P that is capable of giving a relative binding capacity BCrelative≤0.05 or ≥0.1, wherein
- BC
- relative
- =
- R
- P
-
- 1
-
- z
- -
- R
- P
-
- 2
-
- z
- R
- P
-
- 2
-
- z
- -
- R
- P
-
-
- 1
-
- d
- -
- R
- P
-
-
- 2
-
- d
- R
- P
-
-
- 2
-
- d
- (i) wherein where the peptide P comprises a ZIKV-derived peptide,
- RP1z=response of a ZIKV-induced antigen binding protein with peptide P,
- RP2z=response of the ZIKV-induced antigen binding protein with a corresponding peptide C comprising a DENV-derived peptide having a sequence homologous to the peptide P,
- RP1d=response of a DENV-induced antigen binding protein with the peptide P,
- RP2d=response of the DENV-induced antigen binding protein with the corresponding peptide C comprising a DENV-derived peptide having a sequence homologous to the peptide P,
- R
- P
-
- 1
-
- z
- -
- R
- P
-
- 2
-
- z
- R
- P
-
- 2
-
- z
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- ZIKV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- ,
-
- R
- P
-
- 1
-
- d
- -
- R
- P
-
- 2
-
- d
- R
- P
-
- 2
-
- d
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- DENV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- ,
- or
- (ii) wherein where the peptide P comprises a DENV-derived peptide,
- RP1z=response of a ZIKV-induced antigen binding protein with a corresponding peptide C comprising a ZIKV-derived peptide having a sequence homologous to the peptide P,
- RP2z=response of the ZIKV-induced antigen binding protein with the peptide P,
- RP1d=response of a DENV-induced antigen binding protein with the corresponding peptide C comprising a ZIKV-derived peptide having a sequence homologous to the peptide P,
- RP2d=response of the DENV-induced antigen binding protein with the peptide P,
- R
- P
-
- 1
-
- z
- -
- R
- P
-
- 2
-
- z
- R
- P
-
- 2
-
- z
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- ZIKV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- ,
-
- R
- P
-
- 1
-
- d
- -
- R
- P
-
- 2
-
- d
- R
- P
-
- 2
-
- d
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- DENV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- .
(canceled)
A kit for identifying a flavivirus infection selected from Zika virus, dengue virus and combination thereof in a subject, the kit comprising a peptide P that is capable of giving a relative binding capacity BCrelative≤0.05 or ≥0.1, wherein
- BC
- relative
- =
- R
- P
-
- 1
-
- z
- -
- R
- P
-
- 2
-
- z
- R
- P
-
- 2
-
- z
- -
- R
- P
-
-
- 1
-
- d
- -
- R
- P
-
-
- 2
-
- d
- R
- P
-
-
- 2
-
- d
- wherein where the peptide P comprises a ZIKV-derived peptide,
- RP1z=response of a ZIKV-induced antigen binding protein with peptide P,
- RP2z=response of the ZIKV-induced antigen binding protein with a corresponding peptide C comprising a DENV-derived peptide having a sequence homologous to the peptide P,
- RP1d=response of a DENV-induced antigen binding protein with the peptide P,
- RP2d=response of the DENV-induced antigen binding protein with the corresponding peptide C comprising a DENV-derived peptide having a sequence homologous to the peptide P,
- R
- P
-
- 1
-
- z
- -
- R
- P
-
- 2
-
- z
- R
- P
-
- 2
-
- z
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- ZIKV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- ,
-
- R
- P
-
- 1
-
- d
- -
- R
- P
-
- 2
-
- d
- R
- P
-
- 2
-
- d
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- DENV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- ,
- wherein where the peptide P comprises a DENV-derived peptide,
- RP1z=response of a ZIKV-induced antigen binding protein with a corresponding peptide C comprising a ZIKV-derived peptide having a sequence homologous to the peptide P,
- RP2z=response of the ZIKV-induced antigen binding protein with the peptide P,
- RP1d=response of a DENV-induced antigen binding protein with the corresponding peptide C comprising a ZIKV-derived peptide having a sequence homologous to the peptide P,
- RP2d=response of the DENV-induced antigen binding protein with the peptide P,
- R
- P
-
- 1
-
- z
- -
- R
- P
-
- 2
-
- z
- R
- P
-
- 2
-
- z
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- ZIKV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- ,
-
- R
- P
-
- 1
-
- d
- -
- R
- P
-
- 2
-
- d
- R
- P
-
- 2
-
- d
- =
- binding
-
-
- capacity
-
-
- of
-
-
- the
-
-
- DENV-induced
-
-
- antigen
-
-
- binding
-
-
- protein
- .
A composition comprising an isolated peptide or an immune system stimulating composition comprising an isolated peptide, wherein said isolated peptide is selected from the group consisting of:
- SEQ ID NO:
- 7
- (LDEGVEPDDVDCWCNTT);
- SEQ ID NO:
- 36
- (SVEGELNAILEENGVQ);
- SEQ ID NO:
- 38
- (GKSYFVRAAKTNNSFVVD);
- SEQ ID NO:
- 39
- (GDTLKECPLKHRAWNSFL);
- SEQ ID NO:
- 46
- (KVHVEETCGTRGPSLRST);
- SEQ ID NO:
- 49
- (ECTMPPLSFRAK);
- SEQ ID NO:
- 3
- (SFPTTLGMNKCYIQIMDL);
- SEQ ID NO:
- 26
- (GALEAEMDGAKGRLSSGH);
- SEQ ID NO:
- 32
- (FKSLFGGMSWFSQILIGT);
- SEQ ID NO:
- 9
- (YGTCHHKKGEARRSR);
- SEQ ID NO:
- 17
- (PENLEYRIMLSVHGSQHS);
- SEQ ID NO:
- 43
- (REGYRTQMKGPWHSEELE);
- SEQ ID NO:
- 45
- (IRFEECPGTKVHVEETCG);
- SEQ ID NO:
- 58
- (HITEVEPEDIDCWCNLT);
- SEQ ID NO:
- 87
- (QIANELNYILWENNIK);
- SEQ ID NO:
- 89
- (GKAKIVTAETQNSSFIID);
- SEQ ID NO:
- 90
- (GPNTPECPSASRAWNVWE);
- SEQ ID NO:
- 97
- (TVVITENCGTRGPSLRTT);
- SEQ ID NO:
- 100
- (SCTLPPLRYMGE);
- SEQ ID NO:
- 54
- (LFKTASGINMCTLIAMDL);
- SEQ ID NO:
- 77
- (GATEIQNSGGTSIFAGH);
- SEQ ID NO:
- 83
- (YTALFSGVSWVMKIGIGV);
- SEQ ID NO:
- 60
- (TSTWVTYGTCNQAG);
- SEQ ID NO:
- 68
- (YENLKYTVIITVHTGDQH);
- SEQ ID NO:
- 94
- (RPGYHTQTAGPWHLGKLE);
- SEQ ID NO:
- 96
- (LDFNYCEGTTVVITENCG);
- or
- a peptide sharing at least 75% sequence identity thereto;
- or
- a peptide differing by one, two, three or four amino acids thereto;
- or
- portions thereof.
(canceled)
Dependencies are inferred from the claim text.