Automated pathogen and antibody rapid test

A device for detecting primary antibodies to a pathogen or the pathogen comprising:

• a mechanism configured to hold a cartridge configured to receive a test sample, the cartridge comprising:
• a first chamber configured to receive the test sample, the first chamber pre-filled with micromagnetic particles having antigens adapted to bind to antibodies raised against the pathogen immobilized on the micromagnetic particles,
• a first reservoir pre-filled with secondary antibodies labeled with a fluorescent compound, the secondary antibodies adapted to bind to antigens that have bound to antibodies raised against the pathogen, and
• a detection region;
• a mechanism configured to move a magnetic device relative to the cartridge;
• a mechanism configured to move the secondary antibodies from the first reservoir to the first chamber;
• a computer system comprising a processor, memory to store program instructions and data and accessible by the processor, and program instructions stored in the memory and executable by the processor to control the mechanism configured to move the magnetic device to:
• mix the micromagnetic particles with the test sample by moving the micromagnetic particles, so as to facilitate binding of the antigens immobilized on the micromagnetic particles to the antibodies raised against the pathogen,
• mix the micromagnetic particles with the secondary antibodies from the first reservoir in the first chamber, so as to facilitate binding of the secondary antibodies labeled with the fluorescent compound to the antigens that have bound to antibodies raised against the pathogen, and
• move the micromagnetic particles to the detection region after the secondary antibodies labeled with the fluorescent compound have bound to the antigens that have bound to antibodies raised against the pathogen;
• and
• circuitry configured to detect fluorescence of the fluorescent compound in the detection region.