Method and device for detecting hook effect in turbidimetric inhibition immunoassay, and computer readable medium

A method of detecting hook effect in turbidimetric inhibition immunoassay, comprising:

• a) after a sample is mixed with a reaction reagent so that an analyte in the sample starts to react, obtaining an actual measured value Ai at a time point Ti within a predetermined time period T1 to Tn after the reaction starts, where i is an integer from 1 to n, so that a plurality of actual measured values A1 to An are obtained to generate an actual reaction curve for the predetermined time period;
• b) estimating a concentration Ce of the analyte based on the actual reaction curve;
• c) obtaining, according to the estimated concentration Ce of the analyte, a pre-stored reference reaction curve corresponding to the estimated concentration;
• and
• d) comparing distribution information of the actual reaction curve for the predetermined time period with distribution information of the reference reaction curve for the corresponding time period, to determine whether the sample has hook effect.

A blood analysis system, comprising:

• a sampling portion configured to obtain a blood sample and transfer the blood sample to a reaction portion;
• a reagent supply portion configured to store a first reaction reagent and supply the first reaction reagent to the reaction portion as needed;
• the reaction portion comprising a first reaction chamber and configured to mix the blood sample with the first reaction reagent to prepare a first test solution;
• a detection system comprising a light source and a detector for testing the first test solution and configured to obtain measured values of the test solution;
• a controller, which is coupled to the sampling portion, the reagent supply portion, the reaction portion, and the detection system, and is configured to control actions of the sampling portion, the reagent supply portion, the reaction portion, and the detection system;
• and
• a processor, which is coupled to the detection system, wherein
• the processor is configured to:
• obtain, from the detection system, a measured value Ai at a time point Ti within a predetermined time period T1 to Tn after the reaction starts, where i is an integer from Ti to n, so that a plurality of measured values A1 to An are obtained to generate an actual reaction curve for the time period;
• estimate a concentration Ce of the analyte based on the actual reaction curve and a pre-stored calibration curve;
• obtain a pre-stored reference reaction curve corresponding to the estimated concentration according to the estimated concentration Ce of the analyte;
• and
• compare distribution information of the actual reaction curve for the predetermined time period with distribution information of the reference reaction curve for the corresponding time period, to determine whether the sample has hook effect,
• alternatively, the processor is configured to:
• obtain, from the detection system, a measured value Ai at a time point Ti within a time period T1 to Tn after the reaction starts, where i is an integer from 1 to n, so that a plurality of measured values A1 to An are obtained to generate a reaction curve for the time period;
• and perform derivation on the reaction curve A=f(T) within the time period with respect to time T, to obtain a derivative curve, and determine whether the sample has hook effect based on a contour of the derivative curve, and output a determination result to the controller.

The blood analysis system of claim 31, wherein the controller is further configured to:

• control the detection system to stop testing when receiving a result that the current test sample has hook effect;
• control the sampling portion to obtain the sample again and transfer the obtained sample to the first reaction chamber of the reaction portion;
• control the reagent supply portion to supply the first reaction reagent to the first reaction chamber to prepare a second test solution, wherein a dilution factor of the sample in the second test solution is greater than a dilution factor of the sample in the first test solution;
• and
• control the detection system to test the second test solution.