Cation chromatography using predicted elution buffer salt concentration

A chromatography control system comprising a simulation software, the simulation software being configured for performing a method of obtaining pooling borders of a target elution volume comprising a first and a second target protein, the chromatography control system being configured for:

• receiving an optimization criterion and inputting the optimization criterion into the chromatography simulation software, the optimization criterion being a desired property of the target elution volume in respect to the first and second target proteins comprised in the target elution volume;
• computing, using the chromatography simulation software, an elution buffer salt concentration adapted to elute the first and the second target proteins from the chromatography column such that a target elution volume can be obtained that matches the optimization criterion best, the computing comprising computing a plurality of chromatography simulations as a function of multiple different elution buffer salt concentrations;
• computing the pooling borders of the target elution volume as a function of at least the computed salt concentration and the input optimization criterion;
• outputting the computed salt concentration and pooling borders.

A non-transitory computer readable medium storing a computer program, which when executed by a computer system, causes the computer system perform a method of managing a chromatography process such that a target elution volume comprising a first and a second target protein is obtained, the computer program comprising a chromatography simulation software and the computer system further configures to perform

• receiving an optimization criterion being a desired property of a target elution volume in respect to a first and second target proteins comprised in the target elution volume;
• computing, using the chromatography simulation software, an elution buffer salt concentration adapted to elute the first and the second target proteins from the chromatography column such that a target elution volume can be obtained that matches the optimization criterion best, the computing comprising computing a plurality of chromatography simulations as a function of multiple different elution buffer salt concentrations;
• computing the pooling borders of the target elution volume as a function of at least the computed salt concentration and the input optimization criterion;
• and
• outputting the computed salt concentration and/or the pooling borders for enabling a user to control a chromatography system such that a target elution volume comprising the first and the second target proteins in accordance with the optimization criterion is obtained and/or using the computed salt concentration and/or the pooling borders for automatically or semi-automatically controlling a chromatography system such that a target elution volume comprising the first and the second target proteins in accordance with the optimization criterion is obtained.