Systems and methods for chip regeneration

A method for reusing an integrated device to process a sample, the sample comprising analytes, the analytes comprising a biomolecule comprising one or more luminescently labeled molecules, the method comprising:

• loading at least a portion of the sample into a plurality of chambers of the integrated device;
• sampling analytes of the at least the portion of the sample while the analytes are present in the plurality of chambers, wherein the step of sampling comprises collecting a signal emitted by the one or more luminescently labeled molecules indicative of a luminescent lifetime, a luminescent intensity, a wavelength, a pulse duration, and/or an interpulse duration;
• and
• removing the at least the portion of the sample from the plurality of chambers, wherein the removing comprises:
• disrupting a covalent bond between respective coating molecules bound to a surface of the plurality of chambers and coupling moieties, the analytes of the at least the portion of the sample being bound to the coupling moieties.

A method for determining whether a sample is present in one or more chambers of an integrated device, the method comprising:

• loading at least a portion of the sample into the one or more chambers of the integrated device;
• removing the at least the portion of the sample from the one or more chambers of the integrated device;
• delivering excitation light to the one or more chambers of the integrated device;
• collecting signals emitted from the plurality of chambers in response to the excitation light at a photodetection region of the integrated device;
• and
• determining, based on the signals, whether at least some of the at least the portion of the sample is present in the one or more chambers of the integrated device.