Method and kit for the identification of vaccinium myrtillus
AssigneeIndena S.p.A.
InventorsValeria LONGO, Davide BERLANDA
Life of this patent
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Abstract
Disclosed is a method for the identification of Vaccinium myrtillus in a botanical composition and a kit specifically designed for its implementation. The method is based on the detection, using PCR amplification, of nucleic acid fragments within a genomic region of Vaccinium myrtillus.
Claims12
A method for the identification of Vaccinium myrtillus in a botanical composition, which comprises detecting from a sample thereof, by means of PCR-amplification, a V. myrtillus nucleic acid fragment located within the internal transcribed spacer 1, 5.8S ribosomal RNA genomic region and the internal transcribed spacer 2, said method comprising:
- (a) isolating nucleic acids from said sample;
- (b) conducting a real-time PCR on the isolated nucleic acid, using:
- a set of primers selected from the group consisting of:
- (i) SEQ ID NO:5 and SEQ ID NO:6;
- (ii) SEQ ID NO:7 and SEQ ID NO:8;
- (iii) SEQ ID NO:9 and SEQ ID NO:10;
- (iv) SEQ ID NO:11 and SEQ ID NO:12;
- and
- a probe annealing within the nucleic acid region amplified by the primers, said probe consisting of the sequence SEQ ID NO:13;
- (c) determining the presence of the amplification product,
- whereby the detection of the amplification product is indicative of the presence of Vaccinium myrtillus in the botanical composition.
A kit for the identification of Vaccinium myrtillus in a botanical composition, comprising a set of primers selected from the group consisting of:
- (i) SEQ ID NO:5 and SEQ ID NO:6;
- (ii) SEQ ID NO:7 and SEQ ID NO:8;
- (iii) SEQ ID NO:9 and SEQ ID NO:10;
- (iv) SEQ ID NO:11 and SEQ ID NO:12;
- and
- a probe consisting of the sequence SEQ ID NO:13.
The kit of 5, further comprising, in separate containers, a sample of Vaccinium myrtillus nucleic acid suitable for use as positive control and nuclease-free water or buffer solution as negative control.
The kit of 6, further comprising, in separate containers, a sample of Vaccinium myrtillus nucleic acid suitable for use as positive control and nuclease-free water or buffer solution as negative control.
Dependencies are inferred from the claim text.