Multivalent binding composition for nucleic acid analysis

A method for nucleic acid sequencing, said method comprising:

• (a) providing a first nucleotide conjugate comprising (i) a first common core, (ii) a first plurality of labels, and (iii) a first plurality of nucleotides coupled to said first common core;
• (b) contacting a plurality of primed nucleic acid sequences with said first nucleotide conjugate under conditions that preclude phosphodiester bond formation between a first nucleotide of said first plurality of nucleotides and a first complementary nucleotide of said plurality of primed nucleic acid sequences, wherein said first nucleotide of said first plurality of nucleotides stably couples with said first complementary nucleotide in a primed nucleic acid sequence of said plurality of primed nucleic acid sequences;
• (c) detecting a first signal from said first plurality of labels of said first nucleotide conjugate, thereby identifying said first complementary nucleotide of said primed nucleic acid sequence;
• (d) removing said first nucleotide conjugate from said plurality of primed nucleic acid sequences;
• (e) contacting said primed nucleic acid sequence with another nucleotide under conditions that allow phosphodiester bond formation, wherein said second nucleotide is not coupled to said first common core;
• (f) providing a second nucleotide conjugate comprising (i) a second common core, (ii) a second plurality of labels, and (iii) a second plurality of nucleotides coupled said second common core;
• (g) contacting said primed nucleic acid sequence with said second nucleotide conjugate under conditions that preclude phosphodiester bond formation between a second nucleotide of said second plurality of nucleotides and a second complementary nucleotide of said primed nucleic acid sequence, wherein said second nucleotide of said second plurality of nucleotides stably couples with said second complementary nucleotide in said primed nucleic acid sequence;
• and
• (h) detecting a second signal from said second plurality of labels, thereby identifying said second complementary nucleotide of said primed nucleic acid sequence.