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US 2022/0186308 A1Published application
Methods for sequencing nucleic acid molecules
Assignee13.8, Inc.
InventorsChristina FAN, Stephen P.A. FODOR
Life of this patent
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Abstract
Provided herein are methods, compositions, and kits for sequencing nucleic acid molecules of a sample in 3 dimensions (e.g., 3D sequencing).
Claims59
A method of forming a three dimensional (3D) sequencing substrate comprising:
- (a) amplifying a plurality of nucleic acid molecules from a sample in a plurality of partitions, wherein a partition of the plurality of partitions comprises a nucleic acid molecule from the plurality of nucleic acid molecules and a substrate, and wherein amplification couples the nucleic acid molecule from the plurality of nucleic acid molecules or an amplicon thereof to the substrate;
- and
- (b) forming a three dimensional (3D) sequencing substrate from the plurality of partitions.
A method of forming a three dimensional sequencing substrate comprising:
- (a) distributing a plurality of nucleic acid molecules of a sample into a plurality of partitions, wherein a partition of the plurality of partitions comprises a nucleic acid molecule of the plurality of nucleic acid molecules and a substrate of a plurality of substrates;
- (b) coupling the nucleic acid molecule of the plurality of nucleic acid molecules to the substrate of the plurality of substrates in the partition of the plurality of partitions to form a substrate conjugate of a plurality of substrate conjugates, thereby generating the plurality of substrate conjugates in the plurality of partitions;
- and
- (c) forming a three dimensional sequencing substrate from the plurality of partitions.
1 more element
A method of sequencing a plurality of nucleic acid molecules of a sample, the method comprising:
- (a) forming a three dimensional (3D) sequencing substrate from a plurality of partitions, wherein a partition of the plurality of partitions comprises a substrate conjugate, and the substrate conjugate comprises a nucleic acid molecule of the plurality of nucleic acid molecules of the sample coupled to a substrate;
- and
- (b) sequencing the plurality of nucleic acid molecules in the three dimensional sequencing substrate.
A method of sequencing a plurality of nucleic acid molecules of a sample, the method comprising:
- (a) distributing the plurality of nucleic acid molecules of the sample into a plurality of partitions, wherein a partition of the plurality of partitions comprises a nucleic acid molecule of the plurality of nucleic acid molecules and a substrate of a plurality of substrates;
- (b) coupling the nucleic acid molecule of the plurality of nucleic acid molecules to the substrate of the plurality of substrates in the partition of the plurality of partitions to form a substrate conjugate of a plurality of substrate conjugates, thereby generating the plurality of substrate conjugates in the plurality of partitions;
- (c) forming a three dimensional sequencing substrate from the plurality of partitions;
- and
- (d) sequencing the plurality of nucleic acid molecules in the three dimensional (3D) sequencing substrate.
2 more elements
A method of identifying a plurality of nucleic acid molecules of a sample, the method comprising:
- (a) coupling the plurality of nucleic acid molecules to a substrate to produce a plurality of coupled nucleic acid molecules;
- (b) partitioning the plurality of coupled nucleic acid molecules into a plurality of partitions such that each partition comprises a nucleic acid molecule coupled to the substrate;
- (c) forming a three dimensional (3D) sequencing substrate from the plurality of partitions;
- and
- (d) sequencing the plurality of coupled nucleic acid molecules, thereby identifying the plurality of nucleic acid molecules of the sample.
2 more elements
A composition comprising:
- (a) a three dimensional (3D) sequencing substrate;
- (b) a plurality of nucleic acid molecules;
- and
- (c) sequencing reagents.
1 more element
A kit for nucleic acid sequence identification of a sample comprising:
- (a) substrate reagents for forming a three dimensional (3D) sequencing substrate;
- (b) amplification reagents;
- (c) sequencing reagents;
- and
- (d) instructions that direct a user to use the substrate reagents, the amplification reagents, and the sequencing reagents for nucleic acid sequence identification of the sample in the 3D sequencing substrate.
2 more elements
Dependencies are inferred from the claim text.