US 2022/0186307 A1Published application

Image sensor structures and related methods

AssigneesIllumina, Inc., Illumina Cambridge Limited

InventorsLewis KRAFT, Craig Lee HETHERINGTON, Craig M. CIESLA, Michael BUREK, Jeffrey FISHER, Jason BETLEY

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Abstract

An image sensor structure includes an image layer having an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with a light detector. An array of nanowells is disposed over the device stack. Each nanowell is associated with a first light guide of the array of light guides. A first primer set is disposed throughout a first well region of each nanowell. A second primer set is disposed throughout a second well region of each nanowell. The second well region is adjacent the first well region. The first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and a reverse strand cluster of reverse polynucleotide strands in the second well region.

Claims20

Dependent claims

An image sensor structure, comprising:

an image layer comprising an array of light detectors disposed therein;
a device stack disposed over the image layer;
an array of light guides disposed in the device stack, each light guide associated with a light detector of the array of light detectors;
an array of nanowells disposed over the device stack, each nanowell of the array of nanowells associated with a first light guide of the array of light guides, each first light guide associated with a first light detector of the array of light detectors;
a first primer set disposed throughout a first well region of each nanowell;
and
a different second primer set disposed throughout a second well region of each nanowell, the second well region adjacent the first well region at a region interface;
wherein the first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and to attach an adjacent reverse strand cluster of reverse polynucleotide strands in the second well region.

5 more elements

A method comprising:

seeding a first primer set in a first well region of a nanowell of an image sensor structure with a polynucleotide strand;
deactivating a second primer set in a second well region of the nanowell to disable seeding of other polynucleotide strands in the second well region;
amplifying the first polynucleotide strand into a plurality of forward and reverse strands throughout the first well region;
cleaving the reverse strands from the first well region to form a forward strand cluster in the first well region;
activating the second primer set in the second well region to enable seeding and amplification in the second well region;
amplifying from the forward strand cluster of the first well region into the second well region to form a plurality of forward and reverse strands in the second well region;
cleaving the forward strands from the second well region to form a reverse strand cluster in the second well region;
and
sequencing substantially simultaneously the forward strand cluster in the first well region and the reverse strand cluster in the second well region.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/643,454Filed 9 Dec 2021
Priority: 10 Dec 2020
Published: 16 Jun 2022
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/6869
Involving nucleic acids: methods for sequencing
H10F 39/806
Constructional details of image sensors: optical elements or arrangements associated with the image sensors
G01N 21/6454
Individual samples arranged in a regular 2D-array, e.g. multiwell plates: using an integrated detector array
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms