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US 2022/0186304 A1Published application
Sample preparation methods, systems and compositions
AssigneeKarius, Inc.
InventorsTimothy A. BLAUWKAMP, Rene SIT, Igor D. VILFAN
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Abstract
The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.
Claims15
-114. (canceled)
A method for concurrent processing of different nucleic acid forms in a sample comprising:
- (a) providing a sample comprising a first nucleic acid form and a second nucleic acid form;
- (b) denaturing said first nucleic acid form and said second nucleic acid form;
- (c) ligating a first adapter to one end of said first nucleic acid form using a first ligase that has a preference for said first nucleic acid form and ligating a second adapter to one end of said second nucleic acid form using a second ligase that has a preference for said second nucleic acid form, wherein said first adapter and said second adapter each comprise an identifying sequence that is different from each other;
- and
- (d) detecting said ligated nucleic acid forms.
2 more elements
Dependencies are inferred from the claim text.