Method for diagnosing celiac disease based on the level of expression of the ube2l3 gene
AssigneeUniversidad Del Pais Vasco/Euskal Herriko Unibertsitatea
InventorsJosé Ramón BILBAO CATALA, Nora FERNÁNDEZ JIMÉNEZ
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Abstract
The present invention relates to methods for the diagnosis of celiac disease based on the quantification of the expression level of the UBE2L3 gene, as well as of the relative expression of isoform 2 of the UBE2L3 gene with respect to the total expression of the said gene. Likewise, the invention relates to kits with the means needed to perform the diagnosis according to the methods of the invention.
Claims22
An in vitro method for the diagnosis of celiac disease in a subject suspected of suffering celiac disease which comprises quantifying in a sample from said subject the content of:
- (i) the mRNA encoding isoform 2 of the UBE2L3 gene, and/or
- (ii) the total mRNA of the UBE2L3 gene,
- wherein:
- a low level of the mRNA encoding isoform 2 of the UBE2L3 gene with respect to a reference value,
- a high level of the total mRNA of the UBE2L3 gene with respect to a reference value, and/or
- a high relative content of the mRNA encoding isoform 2 of the UBE2L3 gene with respect to the total mRNA of the UBE2L3 gene with respect to a reference value
- is indicative that the subject has celiac disease.
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A kit comprising:
- (i) A probe specifically hybridizing with exon 4 of the UBE2L3 gene,
- (ii) A probe specifically hybridizing with exon 5 of the UBE2L3 gene, and optionally,
- (iii) A probe specifically hybridizing with a constitutive expression gene
- wherein components (i) and (ii) constitute at least 1% of the total of the probes present in the kit.
A method for the diagnosis and treatment of a subject suspected of having celiac disease comprising:
- i) obtaining a sample from a subject suspected of having celiac disease;
- ii) quantifying in said samples the content of:
- a) the mRNA encoding isoform 2 of the UBE2L3 gene, and/or
- b) the total mRNA of the UBE2L3 gene,
- iii) comparing the level of mRNA encoding isoform 2 of the UBE2L3 gene and/or the level of the total mRNA of the UBE2L3 gene with a reference value;
- and
- iv) diagnosing in said subject the presence of celiac disease if:
- a low level of the mRNA encoding isoform 2 of the UBE2L3 gene with respect to a reference value,
- a high level of the total mRNA of the UBE2L3 gene with respect to a reference value, and/or
- a high relative content of the mRNA encoding isoform 2 of the UBE2L3 gene with respect to the total mRNA of the UBE2L3 gene with respect to a reference value
- is detected;
- v) administering to the subject diagnosed with celiac disease a suitable treatment for said disease.
Dependencies are inferred from the claim text.