Molecular Barcode Analysis by Single-Molecule Kinetics
AssigneeQuantum-Si Incorporated
InventorsOmer Ad, Robert E. Boer, Evan McCormack, Brianna Leigh Haining, Thomas Raymond Thurston, Brian Reed
Life of this patent
Estimating the term…
Abstract
Aspects of the disclosure provide methods of determining molecular barcode content based on binding interactions between a barcode recognition molecule and a molecular barcode. In some aspects, the disclosure relates to methods comprising contacting a molecular barcode with a barcode recognition molecule that binds to one or more sites on the molecular barcode, detecting a series of signal pulses, and determining the barcode content based on a barcode-specific pattern in the series of signal pulses.
Claims29
A method comprising:
- contacting a molecular barcode with a barcode recognition molecule that binds to one or more sites on the molecular barcode, wherein the molecular barcode is attached to an analyte comprising a polypeptide;
- detecting a series of signal pulses indicative of binding interactions between the barcode recognition molecule and the molecular barcode;
- and
- determining the identity of the molecular barcode based on a barcode-specific pattern in the series of signal pulses.
(canceled)
9-13. (canceled)
22-31. (canceled)
33-50. (canceled)
A method comprising:
- contacting a molecular barcode with a barcode recognition molecule that binds to one or more sites on the molecular barcode, wherein the molecular barcode is attached to an analyte, wherein an enzyme is bound to the analyte;
- detecting a series of signal pulses indicative of binding interactions between the barcode recognition molecule and the molecular barcode;
- and
- determining the identity of the molecular barcode based on a barcode-specific pattern in the series of signal pulses.
57-112. (canceled)
A method comprising:
- contacting a molecular barcode with a barcode recognition molecule that binds to one or more sites on the molecular barcode, wherein the molecular barcode is attached to an analyte comprising a biomolecule;
- detecting a series of signal pulses indicative of binding interactions between the barcode recognition molecule and the molecular barcode;
- determining the identity of the molecular barcode based on a barcode-specific pattern in the series of signal pulses;
- and
- sequencing the biomolecule by subjecting the biomolecule to sequencing reaction conditions.
114-170. (canceled)
Dependencies are inferred from the claim text.