Novel luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
AssigneePromega Corporation
InventorsLance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
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Abstract
Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
Claims29
-76. (canceled)
An isolated polynucleotide encoding a click beetle red luciferase (CBR) variant polypeptide having at least 90% amino acid sequence identity to SEQ ID NO:
- 1 and consisting of an amino acid substitution at a position corresponding to position 348 of SEQ ID NO:
- 1, wherein the variant CBR polypeptide has at least one of enhanced luminescence, altered light emission wavelength, altered substrate specificity, or a combination thereof, as compared to a CBR polypeptide of SEQ ID NO:
- 1.
An isolated polynucleotide encoding a click beetle red luciferase (CBR) variant polypeptide having at least 90% amino acid sequence identity to SEQ ID NO:
- 1 and comprising amino acid substitutions at a position corresponding to positions 224, 247, 346, and 349 of SEQ ID NO:
- 1, wherein the variant CBR polypeptide has at least one of enhanced luminescence, altered light emission wavelength, altered substrate specificity, or a combination thereof, as compared to a CBR polypeptide of SEQ ID NO:
- 1.
The kit of claim 22 further comprising at least one of:
- (a)
- and
- (b) a buffer reagent.
Dependencies are inferred from the claim text.