Functionalized glass beads, use thereof for capturing microorganisms, and corresponding devices

A glass bead which is functionalized by lysine or polylysine adsorbed on its surface, in which the lysine or polylysine has a molecular weight from about 146 to about 80,300 Da, in particular from about 146 to about 292 Da, of about 292 to about 1460 Da, of about 1460 to about 2920 Da, from about 2920 to about 4380 Da, from about 4380 to about 7300 Da, from about 7300 to about 14,600 Da, from about 14,600 to about 36,500 Da, from about 36,500 to about 58,400 Da, from about 58,400 to about 80,300 Da, in which the polylysine consists of a sequence of 2 to 1000 lysine units, in particular from 2 to 10, from 10 to 20, from 20 to 30, from 30 to 50, from 50 to 100, from 100 to 250, from 250 at 400, from 400 to 550 lysine units.

A device comprising a container containing glass beads which are functionalized by adsorbed lysine or polylysine on their surface, said container being in particular in the form of a column optionally comprising a frit, said column having in particular a volume of about 0.5 mL to about 1 L, and said frit having in particular a porosity less than the size of the used glass beads.

A method for capturing microorganisms comprising a step of bringing a liquid or viscous sample containing said microorganisms into contact with glass beads which are functionalized with lysine or polylysine, under conditions making it possible to create an interaction between said microorganisms and the glass beads, and to obtain said microorganisms captured on the glass beads, in particular

• wherein the proportion of microorganisms from the sample and captured on the glass beads is from 0.001% to 100% for the removal of microorganisms from said sample.