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US 2022/0186281 A1Published application
Method for processing and analysis of viscous liquid biological samples
AssigneeCedars-Sinai Medical Center
InventorsGabriela Guimaraes Sousa Leite, Walter Morales, Stacy Weitsman, Ruchi Mathur, Mark Pimentel
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Abstract
The invention provides for methods and kits for processing viscous biological samples such as intestinal fluids and aspirates. Increased numbers of microorganisms are extracted by the methods and kits of this invention, and thus, DNA extraction and analysis can be performed.
Claims34
A method of increasing the number of microorganisms extracted from a biological sample, comprising:
- adding a reducing agent to the biological sample to produce a mixture;
- and
- centrifuging the mixture to produce a supernatant and a pellet,
- wherein the number of microorganisms extracted is greater than the number of microorganisms extracted without the addition of a reducing agent.
1 more element
A method, comprising:
- adding a reducing agent to a biological sample or biological sample that was stored with a preservation and/or stabilization reagent to produce a mixture;
- and
- centrifuging the mixture to produce a supernatant and a pellet.
27-31. (canceled)
33-37. (canceled)
A kit, comprising:
- a quantity of a reducing agent;
- and
- instructions for using the quantity of the reducing agent to reduce the viscosity of a liquid sample.
(canceled)
(canceled)
Dependencies are inferred from the claim text.