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US 2022/0186277 A1Published application
Nad(p) depot for nad(p)-dependent enzyme-based sensors
AssigneeAbbott Diabetes Care Inc.
InventorsTianmei Ouyang, Cade Fox, Zenghe Liu, Stephen Oja, Benjamin J. Feldman
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Abstract
The present disclosure provides analyte sensors including one or more NAD(P)-dependent enzymes and an internal supply of NAD(P) for the detection of an analyte. The present disclosure further provides methods of using such analyte sensors for detecting one or more analytes present in a biological sample of a subject, and methods of manufacturing said analyte sensors.
Claims18
An analyte sensor comprising:
- (i) an internal supply of NAD(P);
- (ii) a permeable polymer that overcoats the internal supply of NAD(P);
- (iii) at least a first working electrode that is disposed upon a surface of the permeable polymer, wherein the first working electrode is a permeable working electrode;
- (iv) an analyte-responsive active area disposed upon a surface of the first working electrode, wherein the analyte-responsive active area comprises an NAD(P)-dependent enzyme;
- and
- (v) a mass transport limiting membrane permeable to the analyte that overcoats at least the analyte-responsive area.
3 more elements
A method comprising:
- (i) providing an analyte sensor comprising:
- (a) an internal supply of NAD(P);
- (b) a permeable polymer that overcoats the internal supply of NAD(P);
- (c) at least a first working electrode that is disposed upon a surface of the permeable polymer, wherein the first working electrode is a permeable working electrode;
- (d) an analyte-responsive active area disposed upon a surface of the first working electrode, wherein the analyte-responsive active area comprises an NAD(P)-dependent enzyme;
- and
- (e) a mass transport limiting membrane permeable to the analyte that overcoats at least the analyte-responsive area.
- (ii) applying a potential to the first working electrode;
- (iii) obtaining a first signal at or above an oxidation-reduction potential of the first active area, the first signal being proportional to a concentration of a first analyte in a fluid contacting the first active area;
- and
- (iv) correlating the first signal to the concentration of the first analyte in the fluid.
8 more elements
Dependencies are inferred from the claim text.