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US 2022/0186275 A1Published application
Production of Gentisic Acid 5-O-B-D Xylopyranoside
AssigneeFrito-Lay North America, Inc.
InventorsNickolas Anderson, Amanda Waters
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AssigneeFrito-Lay North America, Inc.
InventorsNickolas Anderson, Amanda Waters
Estimating the term…
An expression vector that includes a polynucleotide having a heterologous regulatory element operably linked to a polynucleotide sequence derived from Solanum tuberosum and encoding a xylosyltransferase capable of glycosylating gentisic acid to produce gentisic acid 5-O-β-D xylopyranoside, a transcription template having such a polynucleotide and adapted for in vitro transcription in a cell-free system, a method for producing gentisic acid 5-O-β-D xylopyranoside by culturing a recombinant host cell containing such an expression vector under conditions in which the cell expresses the xylosyltransferase from the polynucleotide, and a method for producing gentisic acid 5-O-β-D xylopyranoside by contacting a composition including gentisic acid and UDP-xylose with a recombinant xylosyltransferase. The recombinant host cell containing such an expression vector can be a bacterial cell, a plant cell, or a fungal cell, an animal cell, or a multicellular organism such as a plant.
A transcription template comprising a polynucleotide that comprises a heterologous regulatory element operably linked to a polynucleotide sequence derived from Solanum tuberosum and encoding a xylosyltransferase, wherein the transcription template is adapted for in vitro transcription in a cell-free system.
An expression vector comprising a polynucleotide comprising a heterologous regulatory element operably linked to a polynucleotide sequence derived from Solanum tuberosum and encoding a xylosyltransferase, the expression vector contained within a recombinant host cell capable of expressing the xylosyltransferase from the polynucleotide and being selected from the group consisting of a bacterial cell, a fungal cell, an animal cell, or a plant cell.
A recombinant host cell comprising a modified level of a xylosyltransferase relative to a wild-type cell of the same taxon, wherein the recombinant host cell is capable of producing gentisic acid 5-O-β-D xylopyranoside when the xylosyltransferase is contacted with UDP-xylose and gentisic acid, and wherein the recombinant host cell comprises a polynucleotide comprising a heterologous regulatory element operably linked to a polynucleotide sequence derived from Solanum tuberosum and encoding the xylosyltransferase.
The multicellular structure of claim 13, wherein the multicellular structure comprises plant tissue.
Dependencies are inferred from the claim text.