Polynucleotide modification methods
AssigneeOxford Nanopore Technologies PLC
InventorsDavid Jackson Stoddart, James White
Life of this patent
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Abstract
The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.
Claims20
1-24. (canceled)
A population of double stranded polynucleotide MuA substrates for modifying a template polynucleotide, wherein each substrate comprises at least one overhang of universal nucleotides.
A population of double stranded polynucleotide MuA substrates for modifying a template polynucleotide, wherein each substrate comprises (i) at least one overhang, and (ii) at least one nucleotide in the same strand as the at least one overhang which comprises a nucleoside that is not present in the template polynucleotide.
Dependencies are inferred from the claim text.