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US 2022/0186272 A1Published application
Strain of trichoderma reesei and culture method and use thereof
OwnerBaode DOU, Guangpeng DOU +4inventors
InventorsBaode DOU, Guangpeng DOU, Zhaobo GAN, Xianbao SHAO, Fanghua LI, Qian DU
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Abstract
The invention relates to a strain of Trichoderma reesei BLCY-007 and its application in the production of xylooligosaccharides.
Claims23
A strain of Trichoderma reesei BLCY-007, which has an accession number:
- CGMCC No. 17970, wherein said accession number was obtained when the strain was deposited on Jun. 14, 2019 in China General Microbiological Culture Collection Center (CGMCC).
A method for preparing xylooligosaccharides, comprising the following steps:
- preparing a premix by crushing and sieving corncobs and adding water thereto;
- subjecting the premix to high-temperature and high-pressure treatment to obtain a crude extract liquid of xylan, wherein the treatment is performed at a treatment temperature from 95° C. to 140° C. and a treatment pressure of 0.05 to 0.25 MPa;
- adjusting the crude extract liquid of xylan to a mass concentration of 4% to 6% and to a pH between 4.2 and 4.8, and performing a microwave treatment to obtain a xylan solution, wherein the microwave treatment is performed at a microwave frequency of 2450 MHz and a treatment temperature from 40° C. to 55° C. for a microwaving time of 10 to 25 minutes;
- adding a xylanase into the xylan solution to perform enzymolysis to obtain a crude xylooligosaccharide solution;
- wherein the xylanase used is a xylanase produced by the following strain:
- said strain is Trichoderma reesei BLCY-007, which has an Accession number:
- CGMCC No. 17970, which was obtained when the strain was deposited on Jun. 14, 2019 in China General Microbiological Culture Collection Center (CGMCC);
- subjecting the crude xylooligosaccharide solution to enzyme inactivation treatment, decolorization treatment, ion exchange treatment, and concentration treatment to obtain an xylooligosaccharide solution.
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Dependencies are inferred from the claim text.