Biosynthetic Cannabidiol Production In Engineered Microorganisms
AssigneeRynetech Bio, Inc.
InventorThomas A. BEARDSLEE
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Abstract
The invention provides engineered biosynthetic pathways that can be used to produce cannabinoids from fatty acids, recombinant microorganisms incorporating such pathways, methods of biosynthetically producing cannabinoids from fatty acids, and cannabinoids so produced.
Claims19
A recombinant microorganism engineered to biosynthetically produce a cannabinoid acid from fatty acids, the recombinant microorganism comprising:
- a. a first engineered biosynthetic pathway to produce hexanoyl-CoA via degradation of fatty acids, preferably monounsaturated fatty acids, which fatty acids are supplied exogenously and/or via de novo fatty acid biosynthesis;
- and
- b. a second engineered biosynthetic pathway to produce a cannabinoid acid from hexanoyl-CoA.
A recombinant microorganism comprising an expression cassette that directs expression of a fatty acyl-CoA oxidase enzyme encoded by a heterologous fatty acyl-CoA oxidase gene, which fatty acyl-CoA oxidase enzyme catalyzes production of hexanoate.
Dependencies are inferred from the claim text.