Method for producing knock-in cell

A method for producing a cell or non-human organism in which a donor sequence is inserted into a genome-editing target region on a genomic DNA, comprising the step of:

• introducing a site-specific nuclease system and donor DNA into a cell or non-human organism, wherein
• the donor DNA is a DNA containing a nucleotide sequence in which a 5′-side homology arm sequence, a donor sequence, and a 3′-side homology arm sequence are arranged in this order from a 5′-side,
• the site-specific nuclease system is a system that targets and cleaves sequences (i) to (iii) of group A or group B below, and the sequences (i) and (ii) of the group A or group B are targeted by the same molecules constituting the site-specific nuclease system, and the sequence (iii) is targeted by other molecules constituting the site-specific nuclease system:
• Group A:
• (i) the 5′-side homology arm sequence
• (ii) A sequence in the genome-editing target region corresponding to the sequence (i)
• (iii) A sequence in the genome-editing target region that is 3′-side downstream from a cleavage site of the sequence (ii) by the site-specific nuclease system;
• and
• Group B
• (i) the 3′-side homology arm sequence
• (ii) A sequence in the genome-editing target region corresponding to the sequence (i)
• (iii) A sequence in the genome-editing target region that is 5′-side upstream from a cleavage site of the sequence (ii) by the site-specific nuclease system.