Flavone 7-o-methyltransferase gene and use for same
AssigneeSuntory Holdings Limited
InventorsNoriko Nakamura, Naoko Okitsu, Yoshikazu Tanaka, Yukihisa Katsumoto
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Abstract
Provided is: a transgenic plant that has a modified flower color; a self- or cross-fertilized descendant of the transgenic plant; or a propagule, a portion of a plant body, tissue, or cells from the transgenic plant or the self- or cross-fertilized descendant of the transgenic plant. The present invention causes both anthocyanin delphinidin and a flavone C-glycoside that is methylated at the hydroxyl group at the 7 position to be present in the cells of a plant.
Claims24
A polynucleotide selected from the group consisting of the following (a) to (e):
- (a) a polynucleotide consisting of the nucleotide sequence listed as SEQ ID NO:
- 34;
- (b) a polynucleotide that hybridizes with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence listed as SEQ ID NO:
- 34 under stringent conditions and encoding a protein having activity of transferring a methyl group to the hydroxyl group at position 7 of flavone C-glycoside;
- (c) a polynucleotide encoding a protein consisting of the amino acid sequence listed as SEQ ID NO:
- 35;
- (d) a polynucleotide encoding a protein consisting of an amino acid sequence that is an amino acid sequence listed as SEQ ID NO:
- 35 having a deletion, substitution, insertion and/or addition of one or more amino acids, and having activity of transferring a methyl group to the hydroxyl group at position 7 of flavone C-glycoside;
- and
- (e) a polynucleotide encoding a protein consisting of an amino acid sequence with at least 90% identity to the amino acid sequence listed as SEQ ID NO:
- 35 and having activity of transferring a methyl group to the hydroxyl group at position 7 of flavone C-glycoside.
A method for creating a transgenic plant with modified flower color, wherein the method comprises a step of causing a delphinidin-type anthocyanin and a flavone C-glycoside to coexist in plant cells, wherein the hydroxyl group at position 7 of the flavone C-glycoside is methylated.
Dependencies are inferred from the claim text.