Bacterial enzymatic conversion of anthracycline chemotherapeutics to reduce toxicity and promote diversity among the intestinal microbiota

A method for preparing a therapeutic composition, the method comprising:

• (a) culturing a bacterial sample obtained from a gastrointestinal tract of a subject in the presence of an anthracycline chemotherapeutic to prepare a cultured sample comprising one or more bacteria that are resistant to the anthracycline chemotherapeutic;
• and (b) formulating the cultured sample for administration to a subject in need thereof as the therapeutic composition, the method optionally further comprising administering the therapeutic composition to the subject in need thereof.

A kit comprising:

• (i) a detoxifying therapeutic agent that detoxifies the anthracycline chemotherapeutic in the gut of the subject;
• and (ii) an anthracycline chemotherapeutic.