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US 2022/0178952 A1Published application
Reagent kit for detecting sex hormone and method for detecting sex hormone using same
AssigneesIndustry-University Cooperation Foundation Hanyang University Erica Campus, Eone Reference Laboratory
InventorsJaebum CHOO, Hyangah CHON, Rui WANG, Sangyeop LEE, Young Ho YOON, Sung Hyun HONG
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Abstract
The present disclosure provides a reagent kit for detecting a sex hormone, which contains a first reagent containing a metal nanoprobe in which a sex hormone and a Raman reporter are immobilized and a second reagent containing a magnetic particle in which an antibody for detecting the sex hormone is immobilized, and a method for detecting a sex hormone using the same.
Claims12
A SERS-based method for detecting a sex hormone comprising the steps of:
- preparing a sample solution comprising a sex hormone;
- preparing a metal nanoprobe in which the sex hormone is bound to a Raman reporter;
- preparing a magnetic particle in which a primary antibody and a secondary antibody for detecting the sex hormone are immobilized;
- adding the metal nanoprobe and the magnetic particle in which the primary antibody and the secondary antibody are immobilized to the sample solution at the same time;
- forming an immunocomplex with the magnetic particle through a competitive immunoreaction of each of the sex hormone in the sample solution and the sex hormone of the metal nanoprobe with the primary antibody immobilized in the magnetic particle;
- separating the magnetic particle in which the immunocomplex is formed using magnetism;
- irradiating a laser light to the separated magnetic particle;
- and
- detecting the sex hormone by measuring a surface-enhanced Raman scattering (SERS) signal after the irradiation of the laser light.
6 more elements
Dependencies are inferred from the claim text.