Prognostic and diagnostic methods for risk of acute kidney injury
AssigneePrevencio, Inc.
InventorsRhonda Fay RHYNE, Craig Agamemnon MAGARET, Grady BARNES, James Louis JANUZZI, John STROBECK
Life of this patent
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Abstract
Compositions and methods are provided for diagnosis and/or prognosis of acute kidney injury risk following medical procedures in a subject. In some embodiments, the method includes measuring and comparing the level of particular proteins to other proteins. In other embodiments, the method includes measuring proteins levels with clinical variable information and comparing this composite with the composite of other protein levels with clinical variable information.
Claims18
A method of determining risk of acute kidney injury in a subject, comprising:
- (i) providing a biological sample from a subject suspected of having a risk of acute kidney injury,
- (ii) applying the biological sample to an analytical device, to
- (a) detect the concentration of at least two protein markers in the sample;
- (b) normalize said concentration of protein markers against a synthetic quantification standard and
- (c) transform the normalized protein marker concentrations;
- wherein the at least two protein markers are selected from those set forth in Table 1;
- (iii) optionally, determining the status of at least one clinical variable or measurement for the subject, wherein the clinical variable or measurement is selected from those set forth in Table 2;
- (iv) calculating a score using an algorithm based on the normalized, transformed protein markers determined in step (ii) and, optionally, the status of the clinical variable or marker determined in step (iii);
- (v) classifying the score as a positive, intermediate, or negative result;
- and
- (vi) determining a prognosis of acute kidney injury risk in a subject as indicated by the score.
A method of administering a therapeutic intervention to a subject having acute kidney injury risk comprising:
- (i) determining the subject's protein marker profile for a panel of protein markers comprising at least two protein markers selected from those set forth in Table 1;
- (ii) optionally, determining the status of at least one clinical variable or measurement for the subject, wherein the clinical variable or measurement is selected from those set forth in Table 2;
- (iii) assigning a score to the subject based on the protein marker profile in (i) and optionally the clinical variable of measurement in (ii) wherein the score is classified as positive, intermediate, and negative, said score algorithmically-derived from the normalized and mathematically transformed concentrations of protein markers in the subject's sample and optionally, the status of at least one clinical variable or measurement;
- and
- (iv) administering to the subject a therapeutic intervention based on the positive, intermediate or negative score.
A method of detecting two or more protein markers in a subject having diabetes type 2 and/or that is suspected of having acute kidney injury risk, the method comprising:
- (i) selecting a subject that has diabetes type 2 and/or that is suspected of having acute kidney injury risk;
- (ii) providing a biological sample from the subject;
- (iii) applying the biological sample to an analytical device, and
- (iv) detecting the concentration of at least two protein markers from Table 1.
-24. (canceled)
A panel for the prognosis of acute kidney injury comprising target-binding agents that bind at least two protein markers selected from those listed in Table 1, a synthetic standard, and optionally, at least one clinical variable selected from those listed in Table 2.
-33. (canceled)
Dependencies are inferred from the claim text.