Methods for identifying differential histone retention for epigenetic alterations in germline dna of animals
AssigneeWashington State University
InventorMichael K. Skinner
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Abstract
The present disclosure relates to methods of analyzing epigenetic alterations in the germline DNA of animals. More particularly, the present disclosure relates to methods of analyzing histone retention involved in epigenetic alterations as well as the identification and treatment of diseases associated with such epigenetic alterations.
Claims20
A method for preparing a DNA fraction from an animal useful for analyzing histone retention involved in an epigenetic alteration, comprising
- (a) extracting DNA from a germline sample of an animal,
- (b) producing a fraction of the DNA extracted in (a) by selecting DNA comprising histone retention, and
- (c) analyzing the histone retention in the fraction of DNA produced in (b).
A method of identifying a disease or a disease propensity in a male progeny animal, comprising:
- identifying a profile of histone retention DNA in the male progeny animal, wherein the profile is associated with the disease or the disease propensity in the male progeny animal, and wherein the disease or the disease propensity is associated with an epigenetic alteration in germline DNA resulting from contact of the germline DNA of the male progeny animal with one or more toxicants during gestation of the male progeny animal.
The method of claim 15, wherein the male progeny animal is administered a pharmaceutical composition to treat the identified disease or the disease propensity.
The method of claim 17, wherein the profile of histone retention DNA is identified according to a method for preparing a DNA fraction from the male progeny animal comprising
- (a) extracting DNA from a germline sample of the male progeny animal,
- (b) producing a fraction of the DNA extracted in (a) by selecting DNA comprising histone retention, and
- (c) analyzing the histone retention in the fraction of DNA produced in (b).
Dependencies are inferred from the claim text.