Method for detection of viral infections using split enzymes
AssigneeBrigham Young University
InventorJonathon T. HILL
Life of this patent
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Abstract
The composition includes a first construct having a first portion of a protein and a first antigen-recognizing amino acid sequence; and a second construct having a second portion of the protein that catalyzes a reaction when combined with the first portion of the protein and a second antigen-recognizing amino acid sequence. The first and second synthetic constructs include a sulfhydryl group configured such that a disulfide bond is formed between the first and second synthetic constructs when the first antigen-recognizing amino acid sequence and the second antigen-recognizing amino acid sequence bind an antigen.
Claims20
A composition for analyte detection, comprising:
- a first construct comprising a first portion of a protein and a first antigen-recognizing amino acid sequence;
- and
- a second construct comprising a second portion of the protein that catalyzes a reaction when combined with the first portion of the protein and a second antigen-recognizing amino acid sequence,
- wherein the first and second synthetic constructs comprise a sulfhydryl group configured such that a disulfide bond is formed between the first and second synthetic constructs when the first antigen-recognizing amino acid sequence and the second antigen-recognizing amino acid sequence bind an antigen.
A method of detecting an analyte, comprising:
- adding a first construct comprising a first portion of a protein and a first antigen-recognizing amino acid sequence to a solution;
- adding a second construct to the solution, the second construct comprising a second portion of the protein that catalyzes an oxidative reaction when combined with the first portion of the protein and a second antigen-recognizing amino acid sequence;
- and
- optionally adding a substrate to the solution,
- wherein the first and second synthetic constructs comprise a sulfhydryl group configured such that a disulfide bond is formed between the first and second synthetic constructs when the first antigen-recognizing amino acid sequence and the second antigen-recognizing amino acid sequence bind an antigen.
A kit, comprising:
- a first construct comprising a first portion of a protein and a first antigen-recognizing amino acid sequence;
- and
- a second construct to the solution, the second construct comprising a second portion of the protein that catalyzes a reaction when combined with the first portion of the protein and a second antigen-recognizing amino acid sequence,
- wherein the first and second synthetic constructs comprise a sulfhydryl group configured such that a disulfide bond is formed between the first and second synthetic constructs when the first antigen-recognizing amino acid sequence and the second antigen-recognizing amino acid sequence bind an antigen.
Dependencies are inferred from the claim text.