Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
AssigneeCornell University
InventorsFrancis BARANY, Eugene SPIER, Alain MIR
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Abstract
The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection
Claims21
1-79. (canceled)
A kit for identifying presence of one or more target nucleotide sequences in a sample comprising:
- an enzyme having 5′ nuclease activity;
- a ligase;
- and
- one or more oligonucleotide probe sets, each set comprising (a) a first oligonucleotide probe having a first target specific portion, and (b) a second oligonucleotide probe having a second target specific portion, wherein the first and second oligonucleotide probes of a probe set are configured to hybridize adjacent to one another on a target nucleotide sequence with a junction between the first and second oligonucleotide probes, and wherein, in each of the one or more oligonucleotide probe sets, the second target specific portion has an overlapping identical nucleotide at the junction with the first oligonucleotide probe.
Dependencies are inferred from the claim text.